The Breeding Blog

I created this blog for two reasons:
To serve as my memory, and to share information about captive breeding of marine fish.

Thursday, January 6, 2011

A. polymnus | Saddleback Clownfish at 12 days old

Just a quick update... the Saddlebacks are 12 days old!!

Since my last post, I have lost 2 larvae.  The remaining are growing well.  There are approximately 6 - 8 that have gone through metamorphosis... Pretty exciting stuff  :)

At this age, they are also fed brine shrimp nauplii and some Otohime larval feed.  I continue with my daily water changes with fresh salt mix aerated for 24 hours.

I caught a quick video clip before cleaning their tank (scraping the sides clean, siphoning out water, rotifers, brine, etc) and adding more RotiGreen & enriched rotifers.  I figured it would be easier to see... it didn't come out too bad.  You can see 2 of the Saddlebacks that have gone through meta.


Tuesday, December 28, 2010

A. polymnus larvae

Today marks 3 days post hatch (DPH) of the A. polymnus (Saddleback clownfish).  As of today, I have approximately 50 - 60 larvae remaining. 

They suffered a large die off the morning after hatch.  My guess, this could be one of two reasons:
  1. The tile was left in too long (overnight).  I left the tile in the hatching tank after most of the larvae had hatched hoping the remaining few would have hatched by morning.  Leaving the tile in there for that length of time could have created "dead" spots where the aeration didn't make it through to the rest of the tank.  ??  I did notice that once I removed the tile, the larvae became more active in the water column.
  2. I added too much RotiGreen Omega all at once.  When I added the Roti Green, I remember squeezing the pipette a bit to hard and put too much in the tank.  I had planned on adding as much as I usually do when adding it to my other larvae tanks however, this was a new pipette that seemed to be thicker than the others.  So, I applied too much pressure.
 Whereas I have heard the A. polymnus larvae are sensitive to water quality, I decided to start small water changes 2 DPH.  I siphoned out 50% of their water without the use of a rotifer sieve and started a slow drip of a mixture of 50% parent water and 50% 24 hour old fresh mix of saltwater.  The rotifers that were allowed to be removed with siphoning out the tank water, was replaced with enriched rotifers.  RotiGreen Omega is added twice daily to keep the tank a slight green.  This helps keep the rotifers nutritious, helps the larvae find the rotifers, and also keeps the larvae in the water column off the sides of the tank.

When looking at the larvae through my jewelers loop (poor man's microscope), the gut of larvae is full and silver.  The silver coloring of the gut indicates they are capturing the rotifers nicely.  At this age (3 DPH) their head and body are mainly silver with their tails black. 

A. polymnus larvae 2 DPH


That is all I have to report at this time.

Since I am new to blogging, I hope the information I provide becomes better with time.  If you have any questions, please ask.

Thank you CaribSea!

A big thank you to CaribSea (and SaltwaterStan) for all the fantastic products that will be put to good use on my breeding system!

I received this today.... 


A full pallet of Primo Reef Rock, Sand, Mineral Mud, and additives!  This is a wonderful Christmas present for my fish and I.  :)

Sunday, December 26, 2010

My Amphiprion polymnus - A. polymnus - Saddleback Clownfish have Spawned!

After waiting patiently, my pair of A. polymnus - Saddleback Clownfish finally spawned on Friday, December 17, 2010.

The Saddleback pair are approximately 2.5 years old, which from what I understand is the age they must attain before they spawn.  The female is 5 - 5.5" and the male is 3.5 - 4".  Although they are not considered the black variant due to their mid stripe not fully extending down the length of their side, they are completely black.  The pair resides in our 40 gallon refugium of our reef / frag system.  I placed them there because I ran out of room in my broodstock system where my breeding fish reside.  :)

Two to three weeks before spawning, the female starting digging in the deep sand bed of the refugium where they are located.  Her digging was to such an extent, she would cloud the water of our 800+ gallon reef / frag system.  On the days she was most actively digging, there were times we could barely see in the 285 gallon display tank.  Those were the days my husband thought about "fishing" for Saddlebacks!

To my delight, at 5:00 PM of the 17th, while feeding my broodstock and our reef system, I noticed the female laying eggs on one of the tiles I placed in the refugium many months ago.  The pair of Saddlebacks spawned for approximately two hours.  After they finished spawning, the female immediately started fanning the eggs with the male lagging behind in his duties.  Every so often, he would fan them and then swim away.  As with most first time spawners, the egg mass was small compared to the amount laid from "veteran" spawning fish.  I would guesstimate there were 300 - 400 eggs whereas, the Saddleback generally lay approximately 1,000 eggs.

Since laying the eggs, the first time spawning female tended to the eggs faithfully while the male (also first time spawner) was vacationing on one of his many anemones.  After the female fanned the eggs, she would go find the male and chase him over to the eggs until he started fanning.  I am sure they both will get better at tending to their eggs in time (namely, the male).

On Saturday, December 25th, he night the eggs were due to hatch, I assembled the rearing tank:
  • 5 gallon tank - all sides except for one painted black
  • black towel to cover the one side of the tank that is not painted
  • small heater set to 80F
  • airstone
  • enough water from the parents tank to cover the tile
After setting up the rearing tank (8:00 PM), I immediately transferred the tile to the tank.  The air from the airstone was set to aerate the eggs enough to keep them in constant motion.  I decided at this point to cover the top of the tank with a black towel also since we have some of our frag tanks on reverse lighting; I wanted to be completely dark in the tank to stimulate them to hatch. 

I couldn't help but take a quick peek in on the tank before going to bed.  When I uncovered a corner of the tank, there they were!  Many very small larvae.  Interestingly, the larvae of the Saddlebacks are smaller than those of my A. percula clownfish.  I thought the Saddlebacks would have been larger than the perculas whereas they are a much larger fish.

I was able to get a video of the fish spawning with my iPhone.  Unfortunately, the video is of poor quality.